Fig. 5: Bottom-up and Top-down profiles following VC Lesions.

A Schematic representation of the V1 lesion and expected reorganization of the pathway. The mouse drawing is adapted from Petrucco, L. Mouse head schema (2020), Zenodo (https://doi.org/10.5281/zenodo.3925903), licensed under CC BY 4.0. B Anatomical image illustrating the bilateral V1 lesions. C Histological image showing a V1-lesioned animal brain with decreased cell density in the lesion site. D, F VE of the CF model averaged across CB animals and computed taking as source LGN and LP, respectively, projected on brain slices. H, J CF size averaged across CB animals when LGN and LP are taken as sources, respectively, projected on brain slices. Quantitative analysis of the CF size (normalized to the mean) as a function of cortical depth for HC (green) and for CB animals (yellow) obtained for the connections: LGN → V1 (E); LP → V1 (G); LGN → HVA (I); LP → HVA (K). The gray dots represent the data from each individual animal, and the gray lines represent the parabolic fit per animal. The green or yellow dots and lines represent the median of all animals from each group. L Quadratic Coefficients obtained for the lCF size profile across cortical layers for controls (green) and CB animals (yellow). The black dots represent the parabolic coefficient from each individual animal. The error bars represent the mean values ± standard error of the mean (SEM). The *** represents a p value < 0.001, **p value < 0.01, and *p value < 0.05 resulting from a two-sided F-test between the parabolic coefficients of controls and CB animals. LGN → V1 (F(1,11) = 28.92, p = 0.0003, d = 5.38); LGN → HVA (F(1,11) = 4.87, p = 0.049, d = 1.128) and LP → HVA (F(1,11) = 30.3, p = 0.003, d = 3.17). Average CF size per layer calculated for HC (green) and CB (yellow) animals obtained for the connections: LGN → V1 (M); LGN → HVA (N); LP → V1 (O); LP → HVA (P). The non-normalized lCF sizes are shown in Fig. S15. The black dots represent the parabolic coefficient from each individual animal. The error bars represent the standard error of the mean. A one way ANOVA corrected for multiple comparisons was performed to investigate the differences in CF size across layers between controls CB animals: LGN → V1 LIV (F(1,11) = 8.63, p = 0.01, d = 2.94), LV (F(1,11) = 9.84, p = 0.01, d = 3.14) and LVI (F(1,11) = 5.81, p = 0.04, d = 2.41); LGN → HVA layer VI: F(1,11) = 11.65, p = 0.007, d = 1.97; and LP → HVA Layer IV F(1,11) = 8.55, p = 0.015, d = 1.69; Layer V F(1,11) = 7.33, p = 0.022, d = 1.56. Source Data has been included as a Source Data file.