Fig. 6: Optimal mitophagy is essential for cellular fitness.
a Crystal violet staining of cells with different expression levels of PRKN with or without DELE1 KD following treatment with 1.25 ng/mL oligomycin for 24 and 48 h. b Immunoblots of ATF4, HSPD1, PINK1, LC3 and COX IV in cells with NTC or DELE1 KD cultured in medium with or without glucose for 24 h. p: precursor; m: mature. c mtKeima reporter cells with PRKN OELL or PRKN OEHL, expressing an NTC or DELE1 sgRNA (DELE1 KD) were cultured with or without glucose (supplemented with galactose or not) for 24 h, followed by measurement of mitophagy using flow cytometry. (mean ± s.d., n = 3 independently treated culture wells) Two-way ANOVA test followed by Turkey’s multiple comparisons test (two-sided). **** adjusted p value < 0.0001. d PRKN OEHL WT or PINK1 KO cells with a NTC or a DELE1 sgRNA were cultured without glucose (with or without galactose) for 24 hr, followed by measurement of mitophagy using flow cytometry. (mean ± s.d., n = 3 independently treated culture wells) Two-way ANOVA test followed by Turkey’s multiple comparisons test (two-sided). **** adjusted p value < 0.0001. e Crystal violet staining of cells with different expression levels of PRKN with or without DELE1 KD cultured without glucose in the medium for 24, 48, 72 and 96 96 respectively. Source data are provided as a Source Data file.
