Fig. 2: PEX11 isoforms differentially contribute to plant development and physiology.

a Immunoblots of sucrose-grown seedlings probed with the indicated antibodies. HSC70 is a loading control. The positions of molecular mass markers (in kDa) are shown on the left. The PMDH2 precursor (p) is cleaved into the mature protein (m) after import into the peroxisome lumen. b The percentage of precursor PMDH versus total PMDH from three biological replicates, including the one shown in a. c pex11cde seedlings display IBA-resistant elongation and growth defects that are partially restored by sucrose supplementation. Dark-grown hypocotyl lengths of individual seedlings (dots) from three biological replicates are shown in three shades of gray, blue, or purple; bars represent means of the replicates (diamonds). d pex11cde seedling growth defects are partially restored by sucrose supplementation. e Representative plants after the transition to flowering. f Representative plants after transfer to soil. g Bars represent mean rosette diameters of individual plants (dots), which included 9, 7, 11, 9, or 4 plants for Wt, Wt reporter, pex14-1, pex11ab, or pex11cde, respectively. h Survival plot of wild-type and mutant plants. 8-day-old seedlings were transferred to soil, and survival was monitored for 35 days. The Mantel-Cox (log-rank) test was used to compare survival curves using pairwise comparison between each genotype compared to the wild-type reporter. p-values were adjusted using Bonferroni correction. The lack of shared letters indicates a significant difference between the distributions. In b, c, and g, letters above bars represent homologous subsets assigned by Tukey’s post-hoc test when one-way ANOVA p-values were <0.05. In c, separate one-way ANOVA tests were performed to compare genotypes at each condition. The lack of shared letters indicates a significant difference between the means. Error bars represent SEM (b, c) or SD (g).