Fig. 2: Increased thiol oxidation causes a reversible decline in DUB activity during mouse brain aging.
From: Oxidative stress causes a reversible decrease of deubiquitylases activity in old vertebrate brains
A Top: Schematic illustrating Ellman’s reagent (DTNB)–based detection of reduced thiol groups, producing the yellow TNB product measurable at 412 nm via spectrophotometry. Bottom: Reduced thiol concentrations in brain lysates from young (3 months) and old (30 months) C57BL/6J male mice (left, N = 8) and young (3 months) and old (22–24 months) C57BL/6J female mice (right, N = 5). Two-tailed unpaired t-test with Welch’s correction. RFU = relative fluorescence units. Data are shown as mean ± SD. B Boxplot of individual active DUB enrichment in old mouse brain lysates with or without 10 mM DTT treatment. 40 DUBs detected under both conditions are shown (N = 3; |average log₂ FC | > 0.58; Q < 0.05; two-tailed Wilcoxon rank-sum test). Data are shown as the median (central line) and the interquartile range (25–75th percentiles, box limits). Whiskers extend to 1.5× the interquartile range, and individual measurements of DUB enrichment are overlaid as dots. C Bar plots showing representative cysteine protease DUB enrichment in old mouse brain lysates treated with or without 10 mM DTT (N = 3; two-tailed unpaired t-test with Welch’s correction). Data are shown as mean ± SD. N refers to the number of biological replicates used in the experiment. Related to Supplementary Data 1. Source data are provided as a Source Data file. Created in BioRender. Sahu, A. (2026) https://BioRender.com/5q3eb5x.
