Fig. 3: DUB inhibition contributes to age-related ubiquitylation signatures.
From: Oxidative stress causes a reversible decrease of deubiquitylases activity in old vertebrate brains
A Schematic illustrating the K-ε-GG antibody-mediated ubiquitylated peptide enrichment and total proteome analysis of iPSC-derived iNeurons treated either with DMSO, 10 µM PR619, or 10 nM Bortezomib (N = 3). B Number of identified ubiquitylated sites in iNeurons treated with the indicated drugs (N = 3; error bars represent the standard deviation from the mean). Data are shown as mean ± SD. C Left: Scatter plot comparing differentially enriched ubiquitylated sites between mouse aging (old vs. young; x-axis; from5) and DUB-inhibited iNeurons (PR619 vs. DMSO; y-axis; this study; N = 3). Right: Quadrant-based Over Representation Analysis (ORA) of the top 10 biological processes. Data includes ubiquitylated site changes with adj.pvals <0.05 (for mouse) and Qvalue < 0.05 (for iNeurons). D Heatmap representing the comparison of average log2 FC of the top 30 enriched ubiquitylated sites per GO term from Quadrant 1 (Q1) in (C) between mouse aging and DUB-inhibited iNeurons. E Heatmap representing the comparison of average log2 FC of the top 30 enriched ubiquitylated sites per GO term from Quadrant 3 (Q3) in (C) between mouse aging and DUB-inhibited iNeurons. For both heatmaps, only differentially enriched sites with adj.pvals <0.05 (for mouse) and Qvalue < 0.05 (for iNeurons) were used. N refers to the number of biological replicates used in the experiment. Related to Supplementary Data 2. Source data are provided as a Source Data file. Created in BioRender. Sahu, A. (2026) https://BioRender.com/5q3eb5x.
