Fig. 4: Temporal study of molecular changes during mouse brain aging.
From: Oxidative stress causes a reversible decrease of deubiquitylases activity in old vertebrate brains
A Schematic illustrating the temporal study of molecular changes during aging in C57BL/6N male and female mouse brains (N = 3–5). B Reduced thiol concentrations in mouse brains across age groups (N = 5 for all male and female groups, except N = 4 for 30 months old females). C DUB activity across age groups (N = 3 for 30 months old females; N = 4 for 3 months old males and 6- and 18-months old females; N = 5 for other groups). D Total ubiquitylated protein levels (N = 3 for females; N = 4 for males). E Proteasome activity (N = 3 for 24 months old males; N = 4 for other male groups and 30 months old females; N = 5 for other female groups). F Schematic illustrating chronic DUB inhibition in iNeurons using increasing concentrations of PR619 and 10 nM bortezomib for 24 h (N = 3-4). G Proteasome activity in iNeurons treated with 7.5 μM PR619, 10 nM bortezomib, or both for 24 h (N = 3). (H) Absolute quantification (AQUA-PRM) of K48-linked ubiquitin chains of iNeurons treated with 7.5 μM PR619, 10 nM bortezomib, or both for 24 h (N = 3). One-way ANOVA was used for analysis in all panels. RFU Relative Fluorescence Units; Dox Doxycycline. Data are shown as mean ± SD in all panels. N refers to the number of biological replicates used in the experiment. Related to Supplementary Data 3. Source data are provided as a Source Data file. Created in BioRender. Sahu, A. (2026) https://BioRender.com/5q3eb5x.
