Fig. 1: Characterization of lipofibroblast marker expression in normal and fibrotic aged Fgf10Cre-ERT2; tdTomatoflox lungs. | Nature Communications

Fig. 1: Characterization of lipofibroblast marker expression in normal and fibrotic aged Fgf10Cre-ERT2; tdTomatoflox lungs.

From: Persistence of alveolar fibroblast-derived ADAMTS4+ cells in a preclinical model of delayed pulmonary fibrosis resolution

Fig. 1: Characterization of lipofibroblast marker expression in normal and fibrotic aged Fgf10Cre-ERT2; tdTomatoflox lungs.The alternative text for this image may have been generated using AI.

a Timeline and schematic of the experimental design. b–e Representative images of in situ hybridization for Tcf21 (white) and immunofluorescence for pro-SFTPC (green) and tdTom (red). The dashed boxes are magnified in the lower panels. f–i Representative images of in situ hybridization for Robo2 (white) and Inmt (green) and immunofluorescence for tdTom (red). The dashed boxes are magnified in the lower panels. j–m Quantification of in situ hybridization and immunofluorescence data. Scale bars: 200 µm. n = 3 per group. Each data point represents one biological replicate. Data are presented as mean ± SEM. Statistical analysis was performed using ordinary one-way ANOVA with Tukey’s multiple comparisons test. * P < 0.05; ** P < 0.01; *** P < 0.001; **** P < 0.0001; ns Not significant. Bleo Bleomycin, DAPI 4′,6-diamidino-2-phenylindole, d.p.i. days post instillation, Fgf10 Fibroblast growth factor 10, Inmt Indolethylamine N-methyltransferase, i.p. Intraperitoneal injection, Robo2 Roundabout guidance receptor 2, Sal Saline, SFTPC Surfactant protein C, Tam Tamoxifen, Tcf21 Transcription factor 21, tdTom tdTomato.

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