Fig. 7: Increased expression of ADAMTS4 in fibrotic human lung disease.
a Integrative UMAP of healthy, IPF, ILD and NSIP. b Frequency of clusters in each patient group. c GSEA of the AF1 signature in IPF, ILD, and NSIP versus healthy datasets. Enrichment scores were calculated using the fgsea algorithm with genes ranked by log2 fold change. P-values were adjusted for multiple comparisons using the Benjamini-Hochberg method, and exact adjusted P values (Adj. P value) are displayed for each plot. d UMAP plots for the indicated genes in healthy, IPF, ILD, and SNIP datasets. e Heatmap of the top DEGs across diseases. Expression values are represented as Z-scores of mean log-normalized counts. Differential expression was determined using a two-sided Wilcoxon Rank Sum test. f Volcano plot showing top upregulated genes in IPF versus healthy. Statistical significance was calculated using a two-sided Wilcoxon Rank Sum test with Bonferroni correction for multiple comparisons. Only adjusted P values are reported. Source data are provided as a Source Data file. ADAMTS4 ADAM metallopeptidase with thrombospondin type 1 motif 4, AF1 Alveolar fibroblasts 1, AF2 Alveolar fibroblasts 2, GSEA Gene set enrichment analysis, HLCA Human lung cell atlas, ILD Interstitial lung disease, IPF Idiopathic pulmonary fibrosis, Meso Mesothelial cells, MyoFB Myofibroblasts, NSIP Non-specific interstitial pneumonia, PeriFB Peribronchial fibroblasts, SMC Smooth muscle cells, SpFB Subpleural fibroblasts.
