Fig. 2: Identification of biosynthetic gene clusters and gene candidates.
From: Nicotine biosynthesis is completed by cryptic activating glucosylation
A Homeologous biosynthetic gene clusters identified in the N. tabacum 2017 genome assembly37 consisting of β-glucosidase (β-GD, blue), reductase (A622, red) and transporter (MATE, yellow) genes. Scaffold/chromosome number is described above the linear genome strand, with numbers at either end representing nucleotide position. Scaffold 884 is unplaced in this assembly but in the Sierro et al. 2024 assembly it is part of chromosome 1675. The gene model between A622 and MATE1 in scaffold 884 (884g0040) encodes a protein that contains a domain with homology to a reverse transcriptase domain (PANTHER ID PTHR33116). B Heatmap of expression of selected nicotine genes, including β-GD and UGT candidates across tissue types. Average expression calculated in each tissue type and values normalized per gene. Number of samples in each category above each column. C Clustered heatmap showing all-by-all correlations (PCC) of nicotine biosynthesis and related genes across 824 samples. TPM values obtained from PEO40. D Root expression levels of selected nicotine biosynthesis genes and UGT candidates following topping treatment, and their correlation (PCC) with A622 responses. Data obtained from Qin et al. 202041. Details of genes available in Supplementary Data 1.
