Fig. 3: In vitro reconstitution of nicotine biosynthesis.
From: Nicotine biosynthesis is completed by cryptic activating glucosylation
A Proposed chemical transformations in nicotine biosynthesis reconstitution. B Products of in vitro cascades. Each row corresponds to an in vitro reaction, with the matrix showing presence/absence (blue/white) of reaction components, alongside UDP-Glc and NADPH. Each column shows extracted ion chromatograms (EICs, m/z ± 0.15). Blue chromatograms are chemically verified standards, scaled for clarity. Red chromatograms highlight key products. MS2 data is available in Supplementary Fig. 3. C Quantification of in vitro cascades. Each row of bars corresponds to an in vitro reaction, with the matrix showing presence/absence (blue/white) of reaction components. Each column of bars shows the peak area of EICs (m/z ± 0.15) corresponding to the masses of the chemical depicted above (left-to-right: 10, 12, 13, 1). Compound identity was validated by standard retention time, and MS2 spectra (Supplementary Fig. 3). Red bars highlight key products. The letters above bars are significant groupings of peak area, determined per chemical (p < 0.05, Tukey post-hoc test, see Supplementary Data 5). The concentration of nicotine produced (from 1 mM of 2) was determined via an external standard curve; error bars show standard error (inset). Bars show mean EIC peak area (n = 3); points are individual replicates; error bars show standard deviation. Source data are provided as a Source Data file.
