Fig. 6: X-ray crystal structures of NaGR (A622) and NicGS (BBLa).
From: Nicotine biosynthesis is completed by cryptic activating glucosylation
A The crystal structure of NaGR bound to NADP+ and nicotinic acid N-glucoside (green net shows unrefined ligand density Fo-Fc at 3 σ). B Proposed mechanism of NaGR: NADPH reduces nicotinic acid N-glucoside-d6 on the re-face, adding a hydride in the pro-R position. C The crystal structure of NicGS with covalently bound FAD and ligand (S)-nicotine glucoside (green net shows unrefined ligand density Fo-Fc at 3 σ). D Proposed mechanism of NicGS, abstracting the pro-S hydride from dihydronicotine glucoside-d6 to yield nicotine glucoside.
