Fig. 8: Identification of pathway intermediates and shunt products in Nicotiana roots.
From: Nicotine biosynthesis is completed by cryptic activating glucosylation
A Metabolite analysis of roots from N. benthamiana wild type, ΔNaGR29 and ΔNicGS31 lines (black) in comparison to standards (blue) (see MS2 verification in Supplementary Fig. 13). Traces are representative extracted ion chromatograms (EICs, m/z ± 0.005) and were normalized to the largest peak in each column; standards were re-scaled to match the largest peak. B Metabolite analysis of roots from N. tabacum var Samsun (black), compared to standards (blue) (see MS2 verification in Supplementary Fig. 14). The red trace is root extract sample spiked with 10 μM nicotine N-glucoside (13) to verify peak identity. Traces are representative EICs (m/z ± 005); standards were re-scaled to match the corresponding peaks in the N. tabacum samples.
