Fig. 5: Modular single-color design enables long-term tracking.

A Setup adapted for single color imaging. This version uses a subset of parts from the dual-color microscope. B 3D rendered model of the setup. C Example image of a C. elegans nematode with the pharyngeal muscle expressing GCaMP8f. D Photobleaching curves (mean ±  s.e.m.) for 1 h of continuous tracking and illumination at 2.78× (N = 5 animals). E Example signals from muscle contractions measured from the GCaMP8f indicator at the beginning and end of the 1 h track for N = 5 animals (top to bottom). F Example 1 h track of a worm. G Ethograms for N = 5 animals for 1 min of data showing speed, feeding, and reversals.