Fig. 4: Depletion or inhibition of eIF5A selectively reduces YFP-CL1 levels. | Nature Communications

Fig. 4: Depletion or inhibition of eIF5A selectively reduces YFP-CL1 levels.

From: Mitochondria serve as a holdout compartment for aggregation-prone proteins hindering efficient degradation

Fig. 4: Depletion or inhibition of eIF5A selectively reduces YFP-CL1 levels.The alternative text for this image may have been generated using AI.

MelJuSo reporter cells YFP-CL1 (a) and UbG76V-YFP (b) were transfected with 20 nM siRNAs against eIF5A for 72 h and analyzed by flow cytometry for YFP fluorescence (n = 5 (a), n = 4 (b) with two control samples per independent replicate, mean ± SD, Kruskal–Wallis test, *P < 0.05, ***P < 0.001, ns: non-significant). MelJuSo reporter cells for YFP-CL1 (c) and UbG76V-YFP (d) were treated with GC7 at the indicated concentrations for 24 h and analyzed by flow cytometry for YFP fluorescence (n = 4 (c) and n = 3 (d) with two control samples per independent replicate, mean ± SD, Kruskal–Wallis test, *P < 0.05, ***P < 0.001, ns: non-significant). e MelJuSo YFP-CL1 cells were transfected with 20 nM siRNAs against eIF5A for 72 h, treated the last 24 h with 10 or 20 µM GC7 and analyzed by flow cytometry for YFP fluorescence (n = 3, mean ± SD, one sample t-test when compared to siCtr or Kruskal–Wallis test when samples were compared within each siEIF5A group, *P < 0.05, **P < 0.01, ***P < 0.001, ns: non-significant).

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