Fig. 2: Nuclear loss lacks left-right symmetry in embryos developing at elevated temperature.
From: Elevated temperature fatally disrupts nuclear divisions in the early Drosophila embryo
A Pie charts show occurrence of blastoderm holes during cellularization in fixed embryos at normal temperature (n = 68 embryos) or elevated temperature (n = 77 embryos): blastoderm hole, a cluster of >10 subcortical nuclei with a corresponding discontinuity in the cortical layer. B Schematic shows various layers of the embryo volume: green, cortical nuclei, <18 µm from embryo surface; magenta, sub-cortical nuclei, >18 µm and <45 µm from embryo surface; yellow, yolk nuclei (vitellophages), >45 µm from embryo surface. C–H Distribution of nuclei in embryos during mid-cellularization (interphase 14), in fixed embryos that were developing at normal temperature (C, F) or elevated temperature that shows either a mild defect (D, G) or a severe defect (E, H). Top/bottom panels, maximum intensity projections in dorsal/ventral halves of the embryos; white/grey dashed lines, midlines drawn based on the egg shape. The voxels are color-coded as in B. Embryo orientation is consistently presented in all panels and is indicated in C. C–E Cortical and subcortical nuclei are shown. Embryos at normal temperature (C) show rarely any gaps, while those at elevated temperature occasionally show small gaps (D) or blastoderm holes (E), indicating discontinuities in the distribution of cortical nuclei, corresponding also with the locations of subcortical nuclei. F–H The corresponding subcortical nuclei are shown separately for improved visibility, along with yolk nuclei. I–N Cross-sectional views long the orange and cyan dashed lines in C–H. Embryo orientation is consistently presented in all panels and is indicated in I. The voxels are color-coded as in B. O, P Blastoderm holes from 11 embryos at elevated temperature were mapped onto a model embryo surface (one blastoderm hole per embryo). Images show projections in dorsal (O) and ventral (P) halves of the model embryo. White/grey dashed lines, midlines drawn based on the egg shape; *, blastoderm hole from embryo in E/H. Scale bars: F–H, 50 µm; I, K, M, 25 µm.
