Fig. 6: VEGFR2 promotes lymphatic sprouting during regenerative lymphangiogenesis. | Nature Communications

Fig. 6: VEGFR2 promotes lymphatic sprouting during regenerative lymphangiogenesis.

From: VEGFR2 is required for VEGF-C–VEGFR3–PI3Kα-mediated sprouting lymphangiogenesis

Fig. 6: VEGFR2 promotes lymphatic sprouting during regenerative lymphangiogenesis.The alternative text for this image may have been generated using AI.

a–c Experimental scheme and whole mount immunofluorescence of the punched ear skin. Boxed areas are magnified below. Arrowheads in LYVE1 staining indicate the spiky vessel ends on the edge of the wound area. Quantification of the spiky vessel ends (b) and vessel area (c) at the wound edge showing the vessel regrowth during one-week period after punching was reduced in Vegfr2-deficient mice and further suppressed upon loss of Vegfr3. Data in (b, c) represent mean ± s.d. (n = 4–6 mice per group as indicated), normalized to control values. Vegfr3-deleted and Vegfr2/Vegfr3 double-deleted (red dots) mice were combined into one group. *p < 0.05, **p < 0.01, ***p < 0.001; One-way ANOVA followed by Tukey’s multiple comparison test. Scale bar: 100 µm (a). Illustration in (a) created using BioRender (https://www.biorender.com).

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