Fig. 2: Fos-TRAP-labeled LH novelty ensemble is activated by pain stimulation. | Nature Communications

Fig. 2: Fos-TRAP-labeled LH novelty ensemble is activated by pain stimulation.

From: Novelty exploration-activated ensemble in the lateral hypothalamus confers analgesic and anxiolytic effects

Fig. 2: Fos-TRAP-labeled LH novelty ensemble is activated by pain stimulation.The alternative text for this image may have been generated using AI.

a Diagram of activity-driven, tamoxifen-enabled Fos-TRAP labeling. b Experimental protocol examining whether Fos-TRAP-eYFP-labeled novelty-activated LH neurons are reactivated by novel object exploration, hind paw mechanical stimulation (von Frey), and formalin injection. c, d Example images and summary showing Fos-TRAP-eYFP selectively labeled LH neurons following tamoxifen administration and novel object exposure (F(2,9) = 22.40, P = 0.0003; n = 4 mice). Representative images and summary showing the overlap between Fos-TRAP-eYFP-labeled LH neurons (green, 1st session of novel object exploration for 1 h) and c-Fos-immunostained neurons (magenta) induced by: e, f a 2nd session of novel object exploration 1-2 weeks later (n = 7 mice); g, h repetitive hind paw stimulation with 2 g von Frey filament for 1 h (n = 7 mice); i, j hind paw formalin injection (n = 7 mice). White arrows indicate the overlap of eYFP- and c-Fos-neurons. Scale bar, 100μm. **P < 0.01, n.s, not significant. One-way ANOVA with Tukey’s post hoc test (c, d). Data are mean ± SEM. Created in BioRender. Zhou, C. (2026) https://BioRender.com/ikcpymj. Source data are provided as a Source Data file.

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