Extended Data Fig. 8: Roles of transporters in supplying pyruvate and phosphoenolpyruvate for amino acid biosynthesis in the chloroplast.
From: Rubisco supplies pyruvate for the 2-C-methyl-d-erythritol-4-phosphate pathway
Concentrations of aromatic (phenylalanine, tryptophan, and tyrosine) and branched chain amino acids (isoleucine, leucine and valine) were analyzed by liquid chromatography – tandem mass spectrometry in leaf extracts from wild-type (WT), cue1, and bass2 plants. Raw peak areas were normalized to the internal standard N-methylglucamine and compared to external standard calibration curves derived from authentic standards. The data are displayed as box plots, with the central line indicating the median and upper and lower bounds representing quartile 3 (75th percentile) and quartile 1 (25th percentile), respectively. Whiskers denote the minima and maxima of the data points. Yellow diamonds indicate mean values (n = 6-7 individual plants). Asterisk indicates significant differences (* P < 0.01, ** P < 0.001, *** P < 0.0001) based on Student’s two-sided t-test.
