Extended Data Fig. 6: HAN does not upregulate WOX5 expression. | Nature Plants

Extended Data Fig. 6: HAN does not upregulate WOX5 expression.

From: A coherent feed-forward loop in the Arabidopsis root stem cell organizer regulates auxin biosynthesis and columella stem cell maintenance

Extended Data Fig. 6

a-b: Representative confocal images of pWOX5:erCFP/wox5-1 (a) and pWOX5:erCFP 35S:HAN-GR/wox5-1 (b) after 8 h of DEX induction. White dotted lines show QC position. n, numbers of roots analyzed. Scale bars: 20 μm. c: Quantification of the CFP intensity measured in the two central QC cells. In pWOX5:erCFP/wox5-1, n = 13; 35S:HAN-GR pWOX5:erCFP/wox5-1, n = 16 roots were analyzed. Error bars denote SD. p = 1.363 × 10−6 by a two-sided Student’s t-test. d: Quantification of the pWOX5:H2B-Tdtomato intensity measured in the two central QC cells of the indicated genotypes. Data shown are means of fluorescence intensity from roots of each indicated genotype. Error bars denote SD. In Col-0, n = 24; han-30, n = 36 roots were analyzed. n.s., not significant, by a two-sided Student’s t-test.

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