Extended Data Fig. 4: RNA-seq analysis of zmice1a endosperms.
From: ZmICE1a regulates the defence–storage trade-off in maize endosperm
a, RT-qPCR analysis of several representative DEGs in the WT and zmice1a endosperms. Data are mean ± s.e.m. (n = 3 biologically independent samples). **, P < 0.01; *, P < 0.05; two-tailed Student’s t-test; for exact P values, see Source Data. b, Heatmap depicting the log2 (fold change) of representative BETL and AL marker genes involved in defence response in the zmice1a endosperms. c, Expression levels of BETL and AL marker genes (SWEET4c and VPP7) in the WT and zmice1a endosperms. Data are mean ± s.e.m. (n = 3 biologically independent samples). ns, not significant; two-tailed Student’s t-test; for exact P values, see Source Data. d, BETL and AL marker genes used in (b) and (c) were functionally annotated. The genes with changed expression levels in the zmice1a endosperms were highlighted in red. e, f, Pathways of IAA synthesis (e) and JA synthesis (f). The expression of enzyme-encoding genes affected by the zmice1a mutation is highlighted in the red boxes.
