Extended Data Fig. 5: CUT&Tag-seq analysis of ZmICE1a.
From: ZmICE1a regulates the defence–storage trade-off in maize endosperm
a, Peak number in two replicates of CUT&Tag-seq data. b, Distribution of ZmICE1a binding region peaks (peak count frequency) corresponding to ± 3000-bp regions flanking the TSS. c, ZmICE1a binding motif identified by MEME-ChIP in the 1000 bp flanking sequences around the genic peak summits. d to f, Peak distribution of ZmICE1a-binding sites for CBF genes (d), COR genes (e), and SBT5.3 (f), visualized using IGV. Rep1 and rep2 indicate two replicates of CUT&Tag-seq data. g, Expression levels of SBT5.3 in maize kernel. Data were collected from the Fu et al. gene expression dataset75. AL, aleurone; BETL, basal endosperm transfer layer; CZ, conducting zone; EAS, endosperm adjacent to scutellum; ESR, embryo-surrounding region; PC, placento-chalazal; PE, pericarp; EM, embryo; SCU, scutellum; SE, starchy endosperm; VE, vitreous endosperm.
