Extended Data Fig. 4: Phenotypes of DF cell layers and wood fibres caused by plt5a plt5b double mutations and WOX4a_pro:PLT5a transgene.

a, Anatomical sections of the 7^th, 8^th and 9^th internodes of 3-month-old WT, plt5a plt5b mutants (#4 and #5) and WOX4a_pro:PLT5a transgenics (#1 and #2). b, Quantification of DF cell layer number at the 7^th, 8^th and 9^th internodes of WT, mutant and transgenic lines corresponding to (a). c, Cellular morphology of fibres isolated from the 10^th internodes of 3-month-old WT, plt5a plt5b mutants (#4 and #5) and WOX4a_pro:PLT5a transgenics (#1 and #2). d and e, The length (d) and width (e) of fibres isolated from the 10^th internodes of WT, mutant and transgenic lines corresponding to (c). In a and c, cross-sections (a) and fibre cells (c) were stained with toluidine blue. In a, DSX domains are indicated in yellow. In b, average values for DF cell layers were determined from 10 radial cell files from cross-sections of the 7^th, 8^th and 9^th internodes, with 5 cross-sections per biological replicate. Date from 6 biological replicates (independent plants) were analyzed, encompassing 30 cross-sections in total. Boxes and whisker plots represent these values. In d and e, 25 fibre cells isolated were quantified for each biological replicate. Six biological replicates (independent plants). The values from all 150 fibre cells from 6 biological replicates were included in box and whisker plots. In b, d and e, boxes show the median and 5^th to 95^th percentile, and whiskers represent the value range excluding outliers. One-way ANOVA analysis followed by Dunnett test for pairwise comparison was performed to evaluate significant differences between WT and mutant or transgenic lines. The average values of 5 cross-sections (b) or 25 fibre cells (d, e) from a biological replicate were used for statistical analyses (n = 6). **, P < 0.01. DF, developing fibre; SXy, secondary xylem; VC, vascular cambium. Scale bars, 30 μm (a) and 100 μm (c).

Source data