Extended Data Fig. 7: The epidermis in the anther lobe is under local tension.
From: Mechanical interactions between tissue layers underlie plant morphogenesis
a, Digital longitudinal cross sections of the anther lobe (top) and tip of connective (bottom) for wild type (left) and spl/nzz (right). Arrows indicate intercellular spaces. b-c, Quantification of ratio between cell growth within the organ plane and growth in cell thickness, per anther region in the epidermis in wild type (b) and spl-1 (c) (n = 13, 49, 77, 134, 175 cell in lobe epidermis, 32, 81, 132, 172, 195 cells in connective epidermis in wild type, 16, 22, 63, 92, 103 cells in lobe epidermis, 26, 47, 127, 182, 205 cells in connective epidermis of the mutant at consecutive time points; 4 independent time-lapse series). In box plots, the lines represent the median and dots represent the mean values, the edges of the boxes define interquartile ranges, the whiskers represent 90% confidence intervals. Asterisks indicate statistical significance determined by two-sided Mann Whitney U test (P < 0.01). d, Confocal images of dissected qua2-1 mutant anther. Insets show details of mid connective (left), tip of connective (top), and lobe (right). Arrows indicate points of cell detachments in the epidermis. Scale bars, 10 μm (a) 20 μm (d).
