Extended Data Fig. 3: Measurements of cell wall thickness and Incipient plasmolysis analysis in ~4 days old anthers.
From: Mechanical interactions between tissue layers underlie plant morphogenesis
a, Representative transmission electron microscopy micrograph of an anther lobe at a stage before lobation. Brackets indicate the layers considered for measurements as shown in Fig. 2e, epidermis (magenta) and inner tissue (green). Insets show segmented lines used to quantify cell wall thickness shown in Fig. 2f. b, Confocal images of anthers, before and after osmotic treatment with solutions of increasing NaCl concentration. The top row shows epidermis while the bottom row shows digital cross sections in the inner tissue. Arrow heads indicate the interstitial spaces created at incipient plasmolysis. Scale bars, 2.5 μm (a), 10 μm (b).
