Extended Data Fig. 4: Obtaining the starting mesh for stiffness reverse-engineering.
From: Mechanical interactions between tissue layers underlie plant morphogenesis
a, 2D meshes extracted from the top-to-bottom and bottom-to-top confocal microscopy scans of the same plasmolyzed sample Cell junctions are highlighted in red. We compute the transformation from one 3D point cloud to the other, M b, Starting mesh and transformed mesh obtained by applying half the transformation M1/2 to the former. The heatmap of volume increase of each cell from the plasmolyzed experimental state to the turgid. c, Pooling of cells into regions. Regions are based on layer, connectivity and volume increase displayed in b. Scale bars, 5 μm.
