Extended Data Fig. 8: The cellular localization of circRNAs and epigenetic modifications of MIR156D region. | Nature Plants

Extended Data Fig. 8: The cellular localization of circRNAs and epigenetic modifications of MIR156D region.

From: Circular RNAs derived from MIR156D promote rice heading by repressing transcription elongation of pri-miR156d through R-loop formation

Extended Data Fig. 8: The cellular localization of circRNAs and epigenetic modifications of MIR156D region.The alternative text for this image may have been generated using AI.

a, RT-qPCR analysis of circRNAs in the nuclear and cytosolic fractions in flag leaves of Nip. The nuclear abundance is set as 1. Data is shown as means ± SD (n = 3). b,c, The levels of H3K27ac (b) and H3K27me3 (c) at the MIR156D locus in wild-type and NIL-sdh. Four pairs of primers (used in Fig. 3a) around the deletion region were used to check the enrichment of H3K27ac or H3K27me3 modifications. The flag leaves were collected for ChIP-qPCR assays. The levels of H3K27ac and H3K27me3 of Actin gene in wild-type was set to 1.0. Three biological replicates were performed. Data is shown as means ± SD (n = 3). A single-sided Student’s t-test was used to generate the P values, *, P < 0.05; **, P < 0.01; ns, not significant.

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