Extended Data Fig. 2: Positioning cloning of SDH.

a, Identification of genomic regions possibly harboring causative mutations using MutMap. The X-axis represents the chromosome positions. The Y-axis represents SNP index for the mutant. The red arrow indicates initial mutation locus interval. b, The diagram of predicted genes between markers RM12584 and RM12590. The boxes represent exons, while the slim lines and bold lines represent introns and regions between genes, respectively. c, PCR analysis of sdh. Primer positions were indicated in Fig. 1d. WT: Gui649; sdh: SDH/sdh obtained from Gui649 x sdh crossing. d, Association analysis between phenotype segregation and DNA deletion in MIR156D in F2 progenies generated from Gui649 and sdh crossing. The analyzed samples were collected based on WT or delayed heading phenotypes and subject to PCR reactions using primer shown in (Fig. 1d).