Extended Data Fig. 4: Identification and over-expression of ciMIR156Ds.
a, Diagram showing the positions of ciMIR156Ds at the MIR156D locus and their validation with divergent primers followed by Sanger sequencing. Dashed lines indicate in electropherogram represent reads matching the back-splicing junction. The typical locations of divergent primers used for circRNA-specific amplification from exon 3 by RT–PCR are shown as grey arrows. b, Schematic diagram of constructs used to over-express ciMIR156D (OE-miR156dcirc) harboring a region from the 1st to the 4th exon of MIR156D. The red lines indicate that miR156 and miR156* were mutated in the construct. Mutated miR156d and miR156* sequences were shown below. c, ciMIR156D transcript levels in 11 independent OE-miR156dcirc transgenic plants detected by RT-PCR. Nip was used as a positive control. Line #1, #4 and #6 were selected for subsequent experiments as OE-miR156dcirc rice.
