Extended Data Fig. 5: Analysis of Al content and ROS production in WT and pp2ch1h2 mutants.
(a) Morin staining in roots of WT and pp2ch1h2 mutant seedlings with or without 20 µM Al treatment for 1 h (bar = 100 µm). (b) Al content in root tips of 1-week-old WT and pp2ch1h2 mutant under Al (25 µM) treatment for 24 h (n = 4 biological repeats). (c) Hematoxylin staining in roots of WT and pp2ch1h2 mutant seedlings with or without 20 µM Al treatment for 1 h (bar = 100 µm). (d) H2DCF-DA staining showing ROS production in root apices of indicated genotypes under control and Al (75 µM) treatment for 15 min (bar = 100 µm). Error bars represent means ± SD. All experiments were repeated at least three independent times with similar results. All data were analyzed by two-tailed unpaired t test (b).
