Extended Data Fig. 6: PP2CH1/2 and ALR1 regulate Al resistance in the same signaling pathway.
(a) Root growth of indicated genotypes under control and 1.2 mM Al treatment for 7 days (bar = 1 cm). (b) Quantification of relative root growth in (a) (n = 20 seedlings). (c, d) Morin and hematoxylin staining in roots of WT, alr1, pp2ch1h2 and alr1pp2ch1h2 seedlings with or without 20 µM Al treatment for 1 h (bar = 100 µm). (e, f) Expression analysis of Al-responsive genes ALMT1 (e) and MATE (f) in roots of 1-week-old seedlings treated with or without 75 µM AlCl3 for 6 h (n = 3 biological repeats). (g) Malate secretion from roots following 24 h control and Al (25 µM) treatments (n = 4 biological repeats). Error bars represent means ± SD. All experiments were repeated at least three independent times with similar results. All data were analyzed by ordinary one-way ANOVA (b, e, f and g).
