Extended Data Fig. 10: Protein alignment of ZmCRK25 with homologous proteins in Arabidopsis.

a, Schematic representation of ZmCRK25 domain composition and organization based on conserved domain analysis. The numbers indicate amino acids positions of the ZmCRK25 protein domains. The positions of the signal peptide (red color), two salt stress response/antifungal domains (stress-antifung), transmembrane (blue color), protein kinase (Pkinase), and low complexity region (purple color) are shown. b, The amino acid sequence of ZmCRK25 was aligned by ClustalW with homologous sequences of CRKs in Arabidopsis: AtCRK25 (AT4G05200.2), AtCRK10 (AT4G23180.1), AtCRK29 (AT4G21410.3). c, Knockout of ZmCRK25. Diagram of genomic structure of ZmCRK25 gene regions edited by CRISPR-Cas9. Bars indicate exons and lines represent introns. Scale bar represents 100 bp.