Extended Data Fig. 4: Analysis of OsECT3/OsECT33WA RNA-binding activities and production of OsECT33WA transgenic plants. | Nature Plants

Extended Data Fig. 4: Analysis of OsECT3/OsECT33WA RNA-binding activities and production of OsECT33WA transgenic plants.

From: Regulation of m6A RNA reader protein OsECT3 activity by lysine acetylation in the cold stress response in rice

Extended Data Fig. 4: Analysis of OsECT3/OsECT33WA RNA-binding activities and production of OsECT33WA transgenic plants.

a-b, Tests of OsECT3- (a) and OsECT33WA- (b) binding activities to RNA probes containing the RRACH motif. For the RNA-EMSA, 4 nmol of the RNA probe were used. RNA probe sequence is 5′-FAM-UCUUUGGXCUGACUUGGACUCUUUA-3′ (X = A/m6A). Protein concentrations were indicated. Two repetitions were performed. c-d, Detection of OsECT3 mRNA and protein levels in WT and COM-E33WA (OsECT3pro::OsECT33WA-Flag/osect3) plants by RT-qPCR (c) and immunoblotting with anti-Flag antibody (d). Bars indicate the mean ± s.d. of three replicates (one-way ANOVA with Tukey’s multiple comparison tests, the significance level was p < 0.05).

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