Extended Data Fig. 8: HDA705 deacetylase activity towards OsECT3 in the HDA705 overexpression plants under cold stress.
a, Detection of relative HDA705 mRNA levels in WT and HDA705 overexpression (OE-HDA705) plants under normal condition. b, Relative expression levels of HDA705 under cold conditions for 0, 6, 12, and 24 h in the OE-HDA705 plants. ACTIN was used as a reference gene, data are presented as mean ± s.d. of three replicates. The significance was calculated using one-way ANOVA with Tukey’s multiple comparison tests in a-b. c, Protein levels of HDA705 treated by cold for 0, 12 h in the OE-HDA705 plants. HDA705-Flag protein was detected with anti-Flag antibody and anti-Actin antibody was used as a loading control. d, Detection of deacetylation level of OsECT3 treated by cold for 0, 12 h in the OE-HDA705 plants. OsECT3 protein was immunoprecipitated with anti-OsECT3 antibody, followed by immunoblotting with anti-OsECT3 or anti-ac-lys antibodies. e, Tests of HDA705 deacetylase activity in 14-day-old WT seedlings under normal and cold conditions. HDA705 protein was isolated by immunoprecipitation using anti-HDA705. HDA705 activities were shown relative to normal conditions (set at 100 %). Error bars are mean ± s.d. from three biological replicates. Significant difference was calculated by the two-tailed, unpaired t-test, the significance level was p < 0.05. Two replicates are shown.
