Extended Data Fig. 10: mRNA m6A enrichment and stability of 4 OsETC3 target genes under normal and cold conditions. | Nature Plants

Extended Data Fig. 10: mRNA m6A enrichment and stability of 4 OsETC3 target genes under normal and cold conditions.

From: Regulation of m6A RNA reader protein OsECT3 activity by lysine acetylation in the cold stress response in rice

Extended Data Fig. 10: mRNA m6A enrichment and stability of 4 OsETC3 target genes under normal and cold conditions.

a. Relative mRNA m6A levels of the 4 cold-repsonsive mark genes by m6A-IP-qPCR in WT, osect3, COM-E3-G, COM-E33WA, COM-E3K471R, and COM-E3K471Q seedlings under normal and cold conditions. External m6A spike-in was used for calibration. Data are presented as mean ± SE, n = 3 independent experiments. The significance was calculated using one-way ANOVA with Tukey’s multiple comparison tests. b-c, The mRNA half-lives of the 4 genes in WT and osect3 seedlings under normal conditions (b) and COM-E3K471Q, COM-E3K471R seedlings under cold conditions (c). Data are presented as mean ± s.d., n = 2 independent experiments, each with 2 technical replicates.

Source data

Back to article page