Fig. 2: WIC inhibits root regeneration.
From: Water availability positions auxin response maxima to determine plant regeneration fates
a, Top: WIC formation in leaf petiole explants of Arabidopsis wild-type and cambial mutants, with the wound site not touching the medium. Bottom: DNRR in the mutants under the same conditions. Numbers on the image indicate how many plants out of 20 showed the phenotype. Normalized WIC area quantifications of wild-type and mutant explants are shown to the right (n = 21, 21, 21, 21 and 24 (left to right) independent explants; *P < 0.05, ***P < 0.001; two-tailed Student’s t-test; box plot shows median (central line), mean (cross), first and third quartiles (top and bottom edges), and minimum and maximum values excluding outliers (whiskers)) b, WIC formation images or quantifications in the cotyledon explants of LBD11-overexpression (OE) or WOX4-OE with the wound site not touching the medium. Mock (DMSO) and 5 µM oestradiol (est) treatments are shown (n = 19, 20, 17 and 15 (left to right) independent explants; ***P < 0.001; two-tailed Student’s t-test; box plot details as in a). c, The frequency of regeneration of WIC and DNRR in the leaf petioles of various mutants with the wound site not in contact with the medium (n = 41, 36, 44, 41 and 45 (left to right) independent explants, mean). d, WIC formation images or quantifications in the leaf explants of LBD11-OE or WOX4-OE when the wound site is in contact with the medium. Mock (DMSO) and 5 µM oestradiol (est) treatments are shown (n = 20 independent explants per genotype; mean). e, Regeneration frequencies of DNRR and WIC in the leaf petiole of LBD16-OE (constitutive) and WOX11-OE (constitutive) with the wound site not touching the medium (n = 20 independent explants per genotype; mean). Dashed lines indicate the wound site in a and b. Scale bar, 200 µm.
