Fig. 5: Water availability shapes spatial auxin response during regeneration.

a, DR5-VENUS auxin-responsive reporter under conditions of high (0.75% agar), intermediate (1.5% agar) and low (2% agar) water availability, with arrowheads indicating auxin maxima in the Arabidopsis leaf petiole. b, The expression of YUC4–GFP under conditions of high (0.75% agar) and low (2% agar) water availability. Dashed green lines mark the leaf vasculature, and arrowheads highlight YUC4–GFP signal. c, The average amount of root regeneration per petiole explant in wild type, yuc2,5,8,9 and yuc1D under high-water conditions (n = 20 independent explants per genotype; *P < 0.05, **P < 0.01, two-tailed Student’s t-test; the box plot shows median (central line), mean (cross), first and third quartiles (top and bottom edges), and minimum and maximum values excluding outliers (whiskers)). d, The normalized WIC area in yuc1D and yuc2,5,8,9 (n = 19, 20 and 15 (left to right) independent explants; **P < 0.01, two-tailed Student’s t-test; box plot details as in c). e, Representative images showing WIC and DNRR in yuc1D and yuc2,5,8,9 mutants under conditions of high (0.75% agar) and low (2% agar) water availability. Scale bars, 100 µm (a and b) and 500 µm (e). The look-up table in b displays the signal intensity range used for a and b. Confocal experiments (a and b) were independently performed three times with similar results.