Extended Data Fig. 9: The weak densities of Psb27 and TEF14-like proteins in the cryo-EM map of TEF30-C and comparison of TEF30-C with the PSII-C associated with Psb27, Psb28 and Psb34. | Nature Plants

Extended Data Fig. 9: The weak densities of Psb27 and TEF14-like proteins in the cryo-EM map of TEF30-C and comparison of TEF30-C with the PSII-C associated with Psb27, Psb28 and Psb34.

From: Roles of multiple TEF30-associated intermediate complexes in the repair and reassembly of photosystem II in Chlamydomonas reinhardtii

Extended Data Fig. 9: The weak densities of Psb27 and TEF14-like proteins in the cryo-EM map of TEF30-C and comparison of TEF30-C with the PSII-C associated with Psb27, Psb28 and Psb34.

a, The unsharpened map of TEF30-C with a low contour level is displayed at the top. The density of TEF30, the mask for Psb27 and the mask for TEF14 are colored in hot pink, blue and light orange, respectively. After the 3D classifications (skipping alignment) with Psb27 or TEF14 masked, the classes of TEF30-Psb27-C and TEF30-PRF1-TEF14-C particles are refined, and the maps are displayed at the bottom. TEF30, Psb27, PRF1 and TEF14 are colored and labeled. b, Superposition of TEF30-C (hot pink) with the PSII-C associated with Psb27, Psb28 and Psb34 (cornflower blue, PDB ID: 7NHP). The two structures are aligned on the D1 subunits. A part of the D1 DE-loop region (Leu223-Glu244) in 7NHP is highlighted in yellow. The overlapping local regions of the binding sites of TEF30 and Psb28 are zoomed in and shown at the bottom.

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