Extended Data Fig. 2: An overall scheme for the cryo-EM data processing of the TEF30-PSII complexes. | Nature Plants

Extended Data Fig. 2: An overall scheme for the cryo-EM data processing of the TEF30-PSII complexes.

From: Roles of multiple TEF30-associated intermediate complexes in the repair and reassembly of photosystem II in Chlamydomonas reinhardtii

Extended Data Fig. 2: An overall scheme for the cryo-EM data processing of the TEF30-PSII complexes.

a, The data processing scheme of the TEF30-C complexes from the TEF30-ox cells. The 3D class chosen for further refinement in each step is circled with light pink rounded rectangles. Besides the major class of TEF30-C particles, the sample also contains minor classes of TEF302-C2 and TEF30-C2S complexes, with 2,863 and 2,670 particles respectively, resulting in low-resolution reconstructions. b, The data processing scheme of the TEF302-C2 and TEF30-C2S complexes. The selected 3D classes of PSII-C dimers were circled with blue rounded rectangles and the selected C2S classes were circled with light purple rounded rectangles. c, The gold standard Fourier shell correlation (GSFSC) curves of the cryo-EM maps of the four different TEF30-PSII complexes with a resolution-cutoff threshold at 0.143. The data was exported from CryoSPARC, and the plot was generated using OriginPro. d, Representative orientational distribution plots for the particle sets of the four different TEF30-PSII complexes. e, Estimation of the local resolution in the cryo-EM maps of the TEF30-PSII complexes.

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