Extended Data Fig. 9: HUG proteins bind to microtubules in vitro and show additional localization along microtubules in pollen tubes. | Nature Plants

Extended Data Fig. 9: HUG proteins bind to microtubules in vitro and show additional localization along microtubules in pollen tubes.

From: Kinesin proteins HUG1 and HUG2 are essential for the formation and transportation of male germ units in Arabidopsis pollen tubes

Extended Data Fig. 9: HUG proteins bind to microtubules in vitro and show additional localization along microtubules in pollen tubes.The alternative text for this image may have been generated using AI.

a, Domain composition of HUG2. Light green box, CC domain; pink box, motor domain. b, Co-sedimentation assay for the binding of HUG2425-1025 with microtubules in vitro. Representative images from three independent experiments. c, Representative images of 8 images showing the dot-like localization of HUG1-mNeonGreen on microtubules (labelled by mCherry-MBD) in pollen tubes. The right panels show overexposed-images inside the white rectangle. d, Relative fluorescent signal intensity along white line in (c). e, Representative images of 3 time-lapse images showing the formation of HUG1-mNeonGreen-labelled dot-like compartments. f, Kymograph along the yellow line in (e). Note that HUG1-mNeonGreen-labelled dot-like compartments were derived from the VN and moved along the microtubules. Scale bar, 5 μm. g, Representative images of 3 images showing the colocalization of HUG2-mNeonGreen with the microtubule network in pollen tubes. h, Relative fluorescent signal intensity along white line in (g).

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