Extended Data Fig. 1: Subcellular localization analysis of 17 kinesin proteins with high expression in pollen. | Nature Plants

Extended Data Fig. 1: Subcellular localization analysis of 17 kinesin proteins with high expression in pollen.

From: Kinesin proteins HUG1 and HUG2 are essential for the formation and transportation of male germ units in Arabidopsis pollen tubes

Extended Data Fig. 1: Subcellular localization analysis of 17 kinesin proteins with high expression in pollen.The alternative text for this image may have been generated using AI.

a, Representative images of 6 images for each kinesin proteins showing the subcellular localization of the 17 kinesins with high expression in pollen. The C-terminaly mNeonGreen-tagged constructs under the native promoters were transformed in the relevant mutant background for the subcellar localization analysis of each kinesin. Notably, two kinesins in the same subclade, referred to as HUG1 and HUG2, exhibited localization in large compartments within pollen tubes. Scale bars, 10 μm. b, c, Representative images of 4 images showing the localization of HUG1-mNeonGreen (b) and HUG2-mNeonGreen (c) surrounding the vegetative nucleus (VN, indicated by the arrows) and the two sperm cells (SCs). DNA in the VN and the SCs were stained with DAPI. Scale bars, 5 μm.

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