Extended Data Fig. 3: HUG1 and HUG2 are highly expressed in pollen.

a, Relative expression levels of HUG1 and HUG2 in different tissues and organs in Arabidopsis. The data were from publicly available RNA seq data (Arabidopsis eFP browser; http://bar.utoronto.ca/). The expression data was normalized with the Gene Chip operating software (GCOS) method with a Target intensity (TGT) and background (Bkg) threshold values of 100 and 20, respectively. The error bars represent means ± s.d. HUG1 and HUG2 are expressed in pollen. b, c, GUS activity assays in transgenic Arabidopsis plants that harbor HUG1 or HUG2 promoter-driven GUS construct. Representative images of 3 images showing GUS signals in inflorescence and pollen. Pollen grains were cultured in vitro for 2.5 h. Scale bars for inflorescence, 0.5 mm. Scale bars for pollen, 10 μm.