Extended Data Fig. 3: TaSnRK2.10-4A confers ABA sensitivity to the ost1-3 mutant.

a, immunoblot analysis of TaSnRK2.10-YFP protein levels in different OE-TaSnRK2.10/ost1-3 transgenic lines using an anti-GFP antibody. b, phenotypic assessment of 7-day-old seedlings of Col-0, ost1-3, and OE-TaSnRK2.10/ost1-3 grown on ½ MS medium with or without 0.5 µM ABA. Scale bars, 2 cm. c, TaSnRK2.10-4A partially restores ABA-insensitive seed germination in ost1-3 mutant. Germination rates were measured using a minimum of 50 seeds per replicate. Error bars indicate mean values ± s.d. of three biological replicates. Asterisks indicate statistically significant differences between samples (One-way ANOVA followed by uncorrected Fisher’s LSD multiple comparisons test, **p < 0.01, ***p < 0.001, ns, no significant difference). d,e, TaSnRK2.10-4A partially restores ABA-insensitive stomatal opening in ost1-3 mutant. Col-0, ost1-3 and OE-TaSnRK2.10/ost1-3 plants were grown on ½ MS medium with or without 10 µM ABA for 7 days, then stomatal aperture was measured following 1 h of light exposure. Scale bars, 20 µm. Error bars indicate mean values ± s.d. (n = 52 independent stomatal). Statistically significant differences between samples are indicated by different letters above the bars (Two-way ANOVA followed by uncorrected Fisher’s LSD multiple comparisons test, p < 0.05).