Extended Data Fig. 8: Contacts within van der Waals distance of residue M/L116.
From: Laboratory evolution of Rubisco solubility and catalytic switches to enhance plant productivity
Contacts (dashed red lines) within van der Waals distance of (a) the modelled M116 in wild type (PDB id 4RUB) and (b) mutant L116 along with (c) a full list of contacts around atoms Cβ and Cγ of Met116 and Leu116. The structure analysis is based on the crystal structure of Rubisco from tobacco (PDB 4RUB). The M116L and A242V substitutions were modeled in their most likely conformations from a library of rotamers53. The modelled residue conformations aligned with the corresponding residues in the structure of the highly CO2-specific non-green algae Griffithsia monilis (PDB id 8BDB)24. The effect of cavity-creating substitutions on protein structure has been discussed in detail in the literature54.
