Extended Data Fig. 3: Generating rbcL plastome transformation plasmids containing direct repeats to facilitate marker excision via homologous recombination within the plastome.
From: Laboratory evolution of Rubisco solubility and catalytic switches to enhance plant productivity
(a) The primers, PCR amplifications and plasmids required for (b) the (i) Golden Gate assembly of the (ii) 4 cloning fragments required to (iii) construct the final transformation vector pLEV-GG-rbcL that contain 405-bp direct repeats (DR) of duplicated regions of the 3’rbcL coding sequence (∆L) and 3’UTR (green T). P, tobacco rbcL promoter + 5′UTR; T, tobacco rps16 terminator; p, T7 promoter; t, T7 terminator.
