Extended Data Fig. 6: MtDELLA1-∆18 stimulates cortex formation upon rhizobium inoculation.
a–c, Cross section images of Medicago WT roots expressing empty vector (a) and pAtSCR::MtDELLA1-∆18–GFP (b and c). d, Root of Medicago della1della2 mutant expressing pAtSCR::MtDELLA1-∆18–GFP. (a, c, d) Roots three weeks after inoculation with transgenic S. meliloti 2011 (Sm2011–GFP). (b) Roots mock treated. C1 to C9, indicate the number of cortical layers 1 to 9. All scale bars, 50 µm. (a-d) for each construct/lines five to eight biological replicates were observed with similar results from two independent experiments. e, Quantification of cortical cell layers in (b) and (d). n, number of independent transgenic plants. The circles represent individual data collected from individual transgenic roots; circles of the same colour represent roots from the same transgenic plants. The bars and error bars are presented as mean values ± s.d. All data were analysed using unpaired two-tailed t-tests. The P value was calculated based on means of independent transgenic plants.
