Extended Data Fig. 8: Loss of Medicago SCR does not affect mycorrhization.
a, Diagram of MtSCR gene structure and Mtscr1-1 Tnt1 insertion position. b, Quantification of mycorrhization level in M. truncatula WT or scr1-1 roots four wpi with R. irregularis according to ref. 50. F%, frequency of analyzed root fragments that are mycorrhized; M%, intensity of infection in the whole roots; m%, intensity of infection in mycorrhized root fragments; A%, arbuscule abundance in entire root fragments; a%, arbuscule abundance in mycorrhized root fragments. n 3 biological repeats. Each circles represent data from independent transgenic plants. The bars and error bars indicate mean values ± s.e.m. All data were analysed using unpaired two-tailed t-tests. c, d, Confocal images showing cortex layers and arbuscules in Medicago WT R108 or Mtscr1-1 transformed roots, six wpi with R. irregularis. The experiments were repeated twice with similar results. (c) Empty vector transformed wild type roots as positive control. (d) pAtSCR::MtDELLA1-∆18–GFP transformed Mtscr1-1 root. Roots six wpi with R. irregularis. ep, epidermis; C1-C9, cortical cell layers; en, endodermis; pc, pericycle. All scale bars, 50 µm. The root cell walls were visualized by SCRI Renaissance 2200 staining and fungal structures with WGA-Alexa Fluor 488. The arrows indicate arbuscules. N > 3 independent biological transgenic lines.
