Extended Data Fig. 9: Phylogenetic tree of Medicago SHR genes and Tnt insertion position of shr mutants.

a, Phylogenetic tree of the Medicago SHR gene family. Medicago SHR gene sequences were obtained from M. truncatula genome v4.0 and v5.0 using blastp of A. thaliana (At) SHR protein sequence. MtSHR1, MtrunA17_Chr5g0401131; MtSHR2, MtrunA17_Chr4g0054071; MtSHR3, MtrunA17_Chr2g0329591. Oryza sativa (Os), Glycine max (Gm) and Solanaceae lycopersicum (Sl) SHR were retrieved from Phytozome v13 (ref. 64,65). The other GRAS family gene members obtained in according to ref. 65. Soybean SHRs were obtained with ref. 66. Neighbor-joining method was used with 2000 bootstraps. b, MtSHR1, MtSHR2 and MtSHR3 gene structure and Tnt1 insertion position of respective mutants.