Extended Data Fig. 2: The expression pattern of AtCASP1::NLS–3xGFP in the Medicago roots.

a, b, pAtCASP1::NLS–3xGFP activity in transformed M. truncatula ecotype Jemalong A17 root tips (a) and mature roots (b). c, d, pAtCASP1::CASP1:NLS–3xGFP activity in transformed M. truncatula ecotype Jemalong A17 root colonized with R. irregularis for four weeks. (c) root meristem. (d) mature root part. The arrowhead indicates arbuscule. e-f, pAtCASP1:: NLS–3xGFP activity in transformed M. truncatula ecotype R108 roots inoculation with (f) or without (e) R. irregularis for four weeks. g, h, pAtCASP1::NLS–3xGFP activity in transformed M. truncatula della1della2 mutant roots inoculation with (h) or without (g) R. irregularis for four weeks. All roots were Clearsee treated and stained with calcofluor white. All scale bars, 50 µm. a–h, The experiments were repeated more than twice with similar results. Each result represents more than five independent biological lines.