Extended Data Fig. 2: Arginine (R) and lysine (K) residues in the NLS are essential for SlSAD8’s nuclear localization. | Nature Plants

Extended Data Fig. 2: Arginine (R) and lysine (K) residues in the NLS are essential for SlSAD8’s nuclear localization.

From: Tomato ripening regulator SlSAD8 disturbs nuclear gene transcription and chloroplast-associated protein degradation

Extended Data Fig. 2: Arginine (R) and lysine (K) residues in the NLS are essential for SlSAD8’s nuclear localization.

a, Schematic representation of different SlSAD8 mutants in the NLS. Red residues indicate point-mutation sites. b, Nuclear localization proportion of SlSAD8 and its mutants. Data are shown as mean ± s.d. of three biological replicates, and differences were assessed using two-tailed t-test (***, P < 0.001). c, Representative subcellular localization images of SlSAD8 and its mutants. Co-expression of the nuclear marker AtHY5-mCherry was used as a reference for nuclear localization. Bars, 20 μm.

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