Extended Data Fig. 5: SAD8 did not interact with three other plastid proteins and was not ubiquitinated by SlSP1. | Nature Plants

Extended Data Fig. 5: SAD8 did not interact with three other plastid proteins and was not ubiquitinated by SlSP1.

From: Tomato ripening regulator SlSAD8 disturbs nuclear gene transcription and chloroplast-associated protein degradation

Extended Data Fig. 5: SAD8 did not interact with three other plastid proteins and was not ubiquitinated by SlSP1.

a-c, LCI assays showed that SAD8 did not interact with ClpR1 (a), SlSPL2 (b), and SlCDC48 (c). d, In vitro ubiquitination assay indicated that SlSP1 did not ubiquitinate SlSAD8. In the scenario where all ubiquitination reaction components were fully supplied (rightmost column), the smear bands indicative of SlSAD8 ubiquitination in anti-His Western blotting remained undetected. All experiments were repeated at three independent times with similar results.

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